4. Genome and proteome of a thermophilic eukaryote for structural analyses of difficult
proteins and complexes.
The properties of thermophilic proteins from prokaryotes have proven to be useful to obtain high resolution structural information of large protein assemblies. The utilization of thermophilic eukaryotic proteins to study the structure and function of conserved eukaryotic molecular machines is currently not possible.
We have recently sequenced to the genome of a thermophilic eukaryotic organism, which gives access to the entire proteome of a thermophilic eukaryote. We now perform studies to test if thermophilic eukaryotic proteins can be engineered from their mesophilic counterparts, which could be a significant advance for eukaryotic protein structural biology. Moreover, the proteome of a thermophilic eukaryote could be used for structural analyses of difficult proteins and protein complexes.
Thomas Monecke, David Haselbach, Piotr Neuman, Emma Thomson, Ed Hurt, Holger Stark,
Achim Dickmanns and Ralf Ficner:
Structures of the nuclear export receptor CRM1 in its free state reveal major structural rearrangements
upon cargo binding
Proc. Natl. Acad. Sci. USA
110, 960-965 (2013).
Vera van Noort, Bettina Bradatsch, Manimozhiyan Arumugam, Stefan Amlacher, Gerd Bange,
Chris Creevey, Sebastien Falk, Daniel R. Mende, Irmgard Sinning, Ed Hurt and Peer Bork:
Consistent mutational paths predict eukaryotic thermostability
BMC Evolutionary Biology
13:7 doi:10.1186/1471-2148-13-7 (2013).
Christoph Leidig, Gert Bange, Jügen Kopp, Stefan Amlacher, Ajay Aravind, Stephan Wickles,
Gregor Witte, Ed Hurt, Roland Beckmann and Irmgard Sinning:
Structural characterization of a eukaryotic co-translational chaperone,
the ribosome-associated complex (RAC)
Nat. Struct. Mol. Biol.
20, 23-28 (2013).
Sébastien Ferreira-Cerca, Vatsala Sagar, Thorsten Schäfer, Anne-Maria Wesseling, Eileen Chai,
Ed Hurt and Nicole LaRonde-LeBlanc:
Structural basis for kinase-activity dependent placeholder role of Rio2 on the evolving pre-40S subunit
Nat. Struct. Mol. Biol.
19, 1316-1323 (2012).
Dieter Kressler, Gert Bange, Yutaka Ogawa, Dagmar Pratte, Bettina Bradatsch, Daniela Roser,
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Jun Katahira, Irmgard Sinning, and Ed Hurt:
Coordinated nuclear transport and assembly of functionally related ribosomal proteins
onto nascent ribosomes by a common import adaptor
Stefan Amlacher, Phillip Sarges, Dirk Flemming, Vera van Noort, Ruth Kunze, Damien Devos,
Manimozhiyan Arumugam, Peer Bork and Ed Hurt:
Insight into structure and assembly of the nuclear pore complex by utilizing the genome
of a eukaryotic thermophile
146, 277-289 (2011).
Gunes Bozkurt, Klemens Wild, Stefan Amlacher, Ed Hurt, Bernhard Dobberstein and Irmgard Sinning:
The structure of Get4 reveals an alpha-solenoid fold adapted for multiple interaction in tail-anchored protein biogenesis
584, 1509-1514 (2010).