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Annual Report 2011-2013

We aim to understand how complicated
macromolecular assemblies - the nuclear pore complex and the ribosomal subunits - are formed in the eukaryotic cell. To reach these goals we apply in vivo cell biology and genetic approaches and in vitro biochemical and structural reconstitution.
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Nuclear pore complexes (NPCs) are the gate- ways facilitating nucleocytoplasmic transport across the nuclear envelope. Although the size of the NPC varies between species, the overall structure is evolutionary conserved from yeast to human. Multiple copies (8, 16, 32) of roughly 30 distinct proteins (nucleoporins, or Nups) build up the NPC, which exhibits an eight-fold rotational symmetry and conspicuous substructures such as the spoke-ring complex, cytoplasmic pore filaments and the nuclear basket visualized by different EM techniques. In the past, several model organisms from lower and higher eukaryotes served to study the huge NPC. Although nucleo- porins from diverse species ...
Hurt Group - Annual Report

Annual Report 2008-2010

Our group performs research with the
goal to elucidate the structure and
function of the nuclear pore complex
and the mechanism of how mRNPs and
ribosomal subunits formin the nucleus
and are exported to the cytoplasm.
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Nuclear pore complexes (NPCs) are the sole mediator of transport between the nucleus and the cytoplasm. Embedded into the doublenuclear membrane, this huge assembly exhibits an eight-foldrotational symmetry with distinct substructures, including the spoke-ring complex, cytoplasmic pore filaments and the nuclear basket.The core structure also contains a central channel through which nucleocytoplasmic transport occurs. The NPC consists of multiple(8, 16 or 32) copies of ~ 30 different proteins named nucleoporins. While a few of them are located asymmetrically on either the nucleoplasmic or cytoplasmic side, most of the ...

Annual Report 2004-2007

To understand the formation and
export of mRNPs and pre-ribosomal
particles through the nuclear pore
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Nuclear pore complexes (NPCs) are huge macromolecular
assemblies embedded in the nuclear envelope that trans-
port proteins and RNAs between the nucleus and cytoplasm.
The NPC, which is composed of ~30 different nucleoporins,
forms an octagonal structure consisting of a central spoke
complex constituting the transport channel, which is em-
braced by a cytoplasmic and nuclear ring. Attached to the
spokering complex are peripheral structures such as the
cytoplasmic pore filaments and the nuclear basket.
Annual Report 2001-2003

In eukaryotic cells, certain steps of
gene expression are restricted to the
nucleus while other steps take place
in the cytoplasm.

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As a consequence of this compartmentalization, an enor-
mous amount of RNA and protein traffic moves through
the nuclear pore complexes (NPCs), whichare embedded
in the nuclear membrane. We study how NPCs are assem-
bled and function in nucleocytoplasmic transport. Moreover,
we investigate the mechanism of nuclear export of mRNA
and pre-ribosomes, and the coupling of their transport to
the machineries that produce and assemble mRNPs ...